Applicants from resource-poor areas including Eastern Europe, Asia, Africa, and South America will be paired with an established mentor. Mentors will be recruited from ISLH and collaborating organizations. This program will consist of 3 parts:
Part 1: Attendance at ISLH 2017 in Honolulu, Hawaii
We have embarked the largest and most comprehensive haematological reference intervals in a multi-ethnic population derived from 2725 Malaysians which has been published and adopted by the local laboratories. In this research project, I would like to utilise these pool of data to establish reference range for advanced and research parameters that are available in two high end analysers (Sysmex XE-5000 and Unicel DxH 800). The advanced red cell parameters could serve as inexpensive surrogate measures of iron restricted erythropoiesis and iron availability especially in the background of inflammation and erythropoietin therapy. It will also be interesting to explore these data to develop formula or algorithms which could aid in the differential diagnosis of thalassemia and iron deficiency anaemia. The usefulness of Cell Population Data (CPD) which is based on 256 channels of histogram data for each VCSn measurement (V, C, MALS, UMALS, LMALS, LALS, AL2) as an additional diagnostic for investigation will be assessed using these data. This will be the first effort to establish a comprehensive reference interval for cell population data that is available in the Unicel DxH 800. Malaysian reference DNAs obtained during this project will also be validated in our customised DNA Microarray (CGH+SNP) chip as it essential to characterise the copy number variations in a Malaysian context.
The need for reference sequence is paramount in characterising copy number variations in a Malaysians in DNA sequence based tests like microarrays. Reference DNAs obtained from three major ethnic groups (Malay, Chinese, and Indian) in Malaysia during this project will be validated using a customised exon-resolution DNA Microarray (CGH+SNP) chip.
Reference ranges are paramount in identifying patients in need of intervention in clinical practice and in research. Complete blood counts (CBC) in particular is a routinely and widely used test. Locally derived reference ranges critical for interpretation of this test in neonates and infants are currently missing in Kenya. Due to the paucity of relevant local reference ranges, international ranges from the Europe and USA are widely used locally and this may lead to erroneous interpretation. The aim of this project is to establish reference ranges for CBC for use in neonates and infants in Kenya. Two hundred and fifty two neonates and Two hundred and fifty two infants will be included in this study. Both sexes will be represented on a 1:1 ratio. A medical officer will examine the infants to determine their clinical status before samples are taken. For those meeting the eligibility criteria, blood samples for CBCs will be collected and analysed using a standard protocol. The reference ranges for CBC parameters will be determined by using methods recommended by Clinical and Laboratory Standards Institute (CLSI) guidelines.
Clinical laboratory practice in Nigeria is fraught with numerous challenges including poor infrastructure, poor funding, old methodologies/manual based assays, low laboratory standards/lack of accreditation, leadership problems, as well as relative unavailability of flow cytometry/molecular assays due to poor local expertise and equipment.
This research seeks to assess the proportion/levels of practice of medical laboratories in Lagos, Nigeria, determine their level of commitment to laboratory standards and quality, audit some existing laboratories with regards to international standards/guidelines, identify the problems/gaps in laboratory service delivery with respect to quality, proffer appropriate solutions/recommendations to regulatory authorities and relevant stakeholders.
Though Flowcytometry has been an integral means of diagnosis in Hematopathology in the western world and even in other countries in Asia itself, it was only recently introduced in our institute. There is thus a lack of adequate expertise and experience in the field in faculties as well as technicians. Through this mentorship program I intend to atleast gain some insight into using and interpreting results through Flowcytometry and where and when possible even guide technicians in the proper handling and formation of framework needed beforehand.
I hope with the help of this mentorship program I am able to impart some of the knowledge I have gained to residents, and even technicians if possible and last but not the least in the proper diagnosis and ultimately treatment of patients in not only Eastern Nepal, but the entire country as well.
I have a burning passion for learning and applying many of the laboratory practice skills especially in the following areas of laboratory hematology: molecular analysis,morphology,platelets and coagulation.Given a choice I will love to acquire skills in carrying out molecular analysis initially in hematological malignancies like AML, ALL,CLL, MPNs and MDS etc . I also have ongoing long term goals of carrying out molecular analysis in coagulation as well as platelet disorders. Last but not the least constantly improving my morphological skills is a lile long ambition and goal.